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目的:探讨2014—2015年江苏省肠道病毒71型(enterovirus 71,EV71)分离株的病毒基因特征。方法:用RD细胞对RT-PCR初筛EV71阳性的标本进行病毒分离,成功分离到的EV71分离株进行VP1区的序列测定和分析。序列分析结果用DNA Star和MEGA 6.0软件进行分析,并与各型参考株进行比较。结果:共分离到35株EV71型毒株,分离阳性率为29.17%。35株EV71分离株VP1区的核苷酸和氨基酸序列同源性分别为93.3%~100.0%和96.6%~100.0%,与EV71的C4a亚型代表株安徽阜阳株的核苷酸和氨基酸序列同源性分别为95.6%~97.9%和97.9%~100.0%;在系统进化树上与C4a亚型代表株相聚在一起,并且存在2个进化分支。结论:2014—2015年江苏省分离的35株EV71毒株均属于C4a亚型;江苏省EV71流行株可能与国内其他省市流行的病毒有着共同的祖先,再各自进化。
Objective: To investigate the viral gene characteristics of enterovirus 71 (EV71) isolates in Jiangsu Province from 2014 to 2015. Methods: RD cells were used to isolate EV71 positive samples from RT-PCR. The isolated EV71 isolates were sequenced and analyzed. Sequence analysis results were analyzed using DNA Star and MEGA 6.0 software and compared with various reference strains. Results: 35 EV71 strains were isolated and the positive rate was 29.17%. The nucleotide and amino acid sequences of VP1 region of 35 isolates of EV71 were 93.3% -100.0% and 96.6% -100.0%, respectively. The nucleotide and amino acid sequences of VP1 region were the same as those of the Fuyang strain of strain C4a of EV71 The origins were 95.6% -97.9% and 97.9% -100.0%, respectively. They were clustered together with C4a subtype representatives in phylogenetic tree, and there were two evolutionary branches. CONCLUSIONS: All the 35 strains of EV71 isolated from Jiangsu Province in 2014-2015 belonged to C4a subtype. The EV71 strains in Jiangsu Province may have a common ancestor with the viruses prevailing in other provinces in China, and then evolved separately.