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目的 研究和克隆新的肝癌凋亡相关基因,探索肝癌发生机制。方法 采用同源筛选、RT-PCR等克隆肝癌凋亡相关基因APG,分析其在肝癌及癌旁组织中表达,测序比对,研究其与肝癌之间相关性。结果 克隆了一个肝癌凋亡相关基因,cDNA全长为563bp。其在肝癌及癌旁组织中序列存在一定差异。50例肝癌中,16%(8/50)为上调表达,84%(42/50)为下调表达(P<0.01)。AGP表达与性别、AFP大小无关(P>0.05),但与肿瘤大小(P<0.05)、HBsAg(P<0.01)、分化程度(P<0.01)、包膜侵犯(P<0.01)、临床分期(P<0.01)、血管癌栓(P<0.01)、癌旁卫星灶(P<0.01)、Ki-67蛋白表达(P<0.01)、细胞凋亡(P<0.05)、P53蛋白表达(P<0.01)密切相关。结论 APG是肝癌凋亡相关的下调基因,其表达与肝癌的某些临床病理特征有关。
Objective To study and clone new genes related to apoptosis of hepatocellular carcinoma and to explore the mechanism of hepatocellular carcinoma. Methods Apoptosis-related gene APG was cloned by homologous screening and RT-PCR. The expression of APG in hepatocellular carcinoma and its adjacent tissues was analyzed by sequencing and compared with that of hepatocellular carcinoma. Results A hepatocarcinoma-related gene was cloned and the full-length cDNA was 563bp. There are some differences in the sequences of HCC and paracancerous tissues. Among 50 cases of hepatocellular carcinoma, 16% (8/50) were up-regulated and 84% (42/50) were down-regulated (P <0.01). The expression of AGP had no correlation with the size of sex and AFP (P> 0.05), but was significantly correlated with tumor size (P <0.05), HBsAg (P <0.01), differentiation (P < (P <0.01), tumor thrombus (P <0.01), adjacent cancer satellite (P <0.01), Ki-67 protein expression <0.01) is closely related. Conclusion APG is a down-regulated gene related to apoptosis of hepatocellular carcinoma, and its expression is related to some clinicopathological features of hepatocellular carcinoma.