论文部分内容阅读
甜菜夜蛾Spodoptera exigua(Hübner)云南晋宁、上海奉贤和江苏六合种群对甲氨基阿维菌素苯甲酸盐抗性为45～437倍,对高效氯氰菊酯抗性为211～555倍,对其它药剂抗性不明显。这3个田间种群3龄幼虫多功能氧化酶、谷胱甘肽S-转移酶和酯酶的活力分别为室内敏感种群的2.7～8.4倍、1.9～8.6倍和1.6～5.7倍。多功能氧化酶抑制剂PBO和酯酶抑制剂DEF对甲氨基阿维菌素苯甲酸盐的增效比为1.2～4.3和1.3～7.7;PBO和DEF对高效氯氰菊酯的增效比为1.8～58和3.6～245;谷胱甘肽S-转移酶抑制剂DEM对这2种药剂均无增效作用。上述结果表明,解毒代谢增强可能是甜菜夜蛾田间种群对甲氨基阿维菌素苯甲酸盐和高效氯氰菊酯的重要抗性机理,与酯酶和多功能氧化酶活性升高有关,与谷胱甘肽S-转移酶活性升高无关。本文的研究结果还表明,对于代谢抗性机理复杂的多抗性田间种群,根据不同解毒酶抑制剂对药剂的增效作用判断不同解毒代谢酶在抗性形成中的作用更加可靠。
Spodoptera exigua (Hübner) The populations of Spodoptera exigua (Hübner) in Yunnan Province, Fengxian of Shanghai and Jiangsu Province were 45- 437 times as resistant to emamectin benzoate and 211- 555 times as resistant to beta-cypermethrin. Pharmacokinetic resistance is not obvious. The activities of multifunctional oxidase, glutathione S-transferase and esterase in 3rd instar larvae in these 3 field populations were 2.7- 8.4 times, 1.9- 8.6 times and 1.6- 5.7 times higher than that of indoor susceptible population respectively. The synergistic ratio of multifunctional oxidase inhibitor PBO and esterase inhibitor DEF to emamectin benzoate was 1.2 ~ 4.3 and 1.3 ~ 7.7. The synergistic ratio of PBO and DEF to alpha-cypermethrin was 1.8 ~ 58 and 3.6 ~ 245; glutathione S-transferase inhibitor DEM no synergistic effect on these two agents. The above results show that enhanced detoxification metabolism may be Beet armyworm field populations of methylamino abamectin benzoate and beta-cypermethrin important resistance mechanism, and esterase and multi-function oxidase activity is elevated, and the glutathione The increase of glycopeptide S-transferase activity was not related. The results of this study also showed that the effect of different detoxification metabolic enzymes on the resistance formation was more reliable based on the synergistic effect of different detoxification enzyme inhibitors on the multi-resistant field populations with complex metabolic resistance mechanisms.