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目的建立芫花挥发性成分的GC指纹图谱,为芫花药材的鉴别及品质控制提供依据。方法采用GC色谱法对所采集的不同地区的芫花药材进行指纹图谱研究,建立GC指纹图谱共有模式,并进行聚类分析和相似度比较。结果 GC指纹图谱对芫花中主要成分均得到较好分离,根据聚类分析和相似度分析处理结果,不同地区的芫花药材指纹图谱相似度存在一定差别。结论该法为芫花药材的质量评价提供科学依据。
Objective To establish a GC fingerprint of the volatile components of Daphne genkwa and provide basis for the identification and quality control of Daphne genkwa. Methods GC fingerprinting was used to study the fingerprints of Daphne genkwa in different regions and the common mode of GC fingerprinting was established, and the clustering analysis and similarity comparison were performed. Results GC fingerprinting showed that the main components in Daphne genkwa were well separated. According to the results of cluster analysis and similarity analysis, there were some differences in fingerprints similarity of different parts of Daphne genkwa. Conclusion This method provides a scientific basis for the quality evaluation of Daphne genkwa.