[Objective] HPLC method was developed to determine the contents of berberine hydrochloride in Shihu Yeguang pills.[Method] C18 column(250 mm×4.6 mm,5 μm) was introduced in this study,acetonitrile-0.34% potassium dihydrogen phosphate-0.17% sodium dodecylsulphate(48:26:26) as the mobile phase,345 nm as the detection wavelength.[Result]The linear relationship of chlorogenic acid was good in the range of 0.042 8-1.154 8 μg,the recovery rate was 99.5% and the RSD was 2.8%.[Conclusion]The method is convenient and accurate that could be applied in quantitative determination of the medicine. [Objective] HPLC method was developed to determine the contents of berberine hydrochloride in Shihu Yeguang pills. [Method] C18 column (250 mm × 4.6 mm, 5 μm) was introduced in this study, acetonitrile- 0.34% potassium dihydrogen phosphate-0.17% sodium dodecylsulphate (48:26:26) as the mobile phase, 345 nm as the detection wavelength. [Result] The linear relationship of chlorogenic acid was good in the range of 0.042 8-1.154 8 μg, the recovery rate was 99.5% and the RSD was 2.8%. Conclusion The method is convenient and accurate that could be applied in quantitative determination of the medicine.