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细胞的非程序合成(UDS)已被广泛用于研究化学物质和物理因子的细胞遗传毒理作用,并已被认为可用作致癌物快速筛选的一种手段。一般常用人成纤维细胞、HeLa细胞和人外周血淋巴细胞,也有用人羊膜细胞为材料。然而这类细胞常缺乏完整的代谢致癌物的酶系统,因而近年来William等建立了大鼠肝原代培养细胞的UDS方法,以图保持致癌物代谢酶系的完整性。然而在肝原代培养过程中,细胞分离后必须先培养24小时,待细胞贴壁后才能用于实验;但是Michalo-
Unprogrammed cell synthesis (UDS) has been widely used to study cytogenetic toxicological effects of chemical substances and physical factors, and has been considered as a means of rapid screening of carcinogens. Commonly used human fibroblasts, HeLa cells and human peripheral blood lymphocytes, but also useful for human amniotic cells. However, these cells often lack complete enzymatic systems for metabolizing carcinogens. In recent years, William et al. established a UDS method for rat primary cultured cells in order to maintain the integrity of carcinogenic metabolic enzymes. However, in the process of primary liver culture, the cells must be cultured for 24 hours after separation, and the cells can be used for experiments after adherence; however, Michalo-