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目的探讨Ghrelin对大鼠胃排空及小肠传输功能的作用。方法制备术后肠动力障碍大鼠模型,观察并比较99mTc-甲基纤维素灌胃后静脉注射Ghrelin、降钙素基因相关肽(CGRP)对胃排空和小肠传输功能的影响。结果 99mTc-甲基纤维素灌胃后静脉注射Ghrelin(20μg/kg),15~60min胃内99mTc-甲基纤维素的残留量明显减少(F=248.13,q=5.15~21.60,P<0.01),且呈显著剂量依赖关系(q=3.15~9.64,P<0.05)。十二指肠给予99mTc-甲基纤维素,静脉注射Ghrelin 15min后,大鼠回肠上1/3内99mTc-甲基纤维素的残留量明显下降(F=16.41,q=10.56,P<0.01),但回肠中1/3内99mTc-甲基纤维素的残留量却明显上升(q=11.44,P<0.01)。大鼠肠梗阻术后的胃内99mTc-甲基纤维素残留量明显高于假手术对照组,即胃排空率显著降低(t=2.78,P<0.05)。静脉注射Ghrelin(4~50μg/kg)15min后,肠梗阻大鼠胃内99mTc-甲基纤维素的残留量较对照组显著减少,且呈显著量效依赖关系(t=3.04~6.22,P<0.05)。静脉注射CGRP 50μg/kg,可明显改善梗阻后胃内99mTc-甲基纤维素的残留量(t=2.31,P<0.05),但该效应明显低于20μg/kg Ghrelin对胃排空作用(t=5.07,P<0.01)。结论 Ghrelin可促进大鼠胃肠排空,改善术后肠梗阻的症状。
Objective To investigate the effect of Ghrelin on gastric emptying and intestinal transit in rats. Methods The rat model of postoperative bowel dysmotility was prepared and the effects of intravenous Ghrelin and CGRP on the gastric emptying and intestinal transit function after 99mTc-methylcellulose administration were observed and compared. Results Ginrelin (20μg / kg) was given intravenously after administration of 99mTc-methylcellulose, and the residual amount of 99mTc-methylcellulose in stomach was significantly decreased after 15 ~ 60min (F = 248.13, q = 5.15-21.60, P <0.01) , And showed a significant dose-dependent relationship (q = 3.15 ~ 9.64, P <0.05). 99mTc-methylcellulose was given to the duodenum. After intravenous injection of Ghrelin for 15min, the residual 99mTc-methyl cellulose in the upper third of the ileum significantly decreased (F = 16.41, q = 10.56, P <0.01) , But the residual amount of 99mTc-methyl cellulose in the middle third of ileum increased significantly (q = 11.44, P <0.01). The residual 99mTc-methylcellulose in the stomach after ileus operation was significantly higher than that in the sham operation control group, that is, the gastric emptying rate was significantly decreased (t = 2.78, P <0.05). After intravenous injection of Ghrelin (4 ~ 50μg / kg) for 15min, the residual amount of 99mTc-methylcellulose in the stomach of rats with ileus decreased significantly compared with the control group (t = 3.04-6.22, P < 0.05). Intravenous injection of CGRP 50 μg / kg significantly improved the residual 99mTc-methylcellulose in the stomach after obstruction (t = 2.31, P <0.05), but the effect was significantly lower than that of 20 μg / kg Ghrelin on gastric emptying = 5.07, P <0.01). Conclusion Ghrelin can promote gastrointestinal emptying in rats and improve the symptoms of postoperative intestinal obstruction.