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目的:用蛋白质组学技术比较重症急性胰腺炎(SAP)与轻症急性胰腺炎(MAP)患者血清的差异蛋白,探讨SAP特异蛋白的表达。方法:血清标本取自10例急性胰腺炎(AP)患者,其中5例SAP,5例MAP,用双向凝胶电泳检测两类患者的蛋白表达,并将这些差异表达蛋白点进行质谱鉴定,Mascot数据库检索。结果:比较双向电泳图谱发现15个差异表达蛋白点,经质谱分析,成功鉴定出10种蛋白质,在SAP患者其中4种表达上调,6种表达下调。数据库查询结果,4个表达上调蛋白分别为补体4A、结合珠蛋白亚型2、结合珠蛋白和载脂蛋白L1;6个表达下调蛋白分别为四连接素、谷胱甘肽过氧化物酶3、丝氨酸蛋白酶抑制剂亚型F1、丛生蛋白亚型-1、转甲状腺素蛋白、丝氨酸蛋白酶抑制剂A1亚型2。结论:SAP患者血清有差异蛋白质表达,这可能和SAP发病机制相关。
OBJECTIVE: To compare the differential proteins in patients with severe acute pancreatitis (SAP) and mild acute pancreatitis (MAP) using proteomics to investigate the expression of SAP-specific proteins. Methods: Serum samples were collected from 10 patients with acute pancreatitis (AP), including 5 cases of SAP and 5 cases of MAP. Two-dimensional gel electrophoresis was used to detect the protein expression of two kinds of patients. The differentially expressed proteins were identified by mass spectrometry. Mascot Database search. RESULTS: Fifteen differentially expressed protein spots were found by two-dimensional gel electrophoresis. Ten proteins were successfully identified by mass spectrometry. Four of them were up-regulated and six of them were down-regulated in SAP patients. According to the results of database search, the four up-regulated proteins were complement 4A, haptoglobin subtype 2, haptoglobin and apolipoprotein L1 respectively; the six down-regulated proteins were tetranectin, glutathione peroxidase 3 , Serine protease inhibitor subtype F1, clusterin subtype-1, transthyretin, serine protease inhibitor subtype A1. Conclusion: The serum of patients with SAP has differential protein expression, which may be related to the pathogenesis of SAP.