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目的探讨肾细胞癌中干细胞关键转录因子Oct4的表达情况及意义,并探讨Oct4的表达同肾细胞癌分型和分期的关系。方法分别采用免疫组织化学对27例和逆转录-聚合酶链反应(RT-PCR)对10例肾细胞癌组织病理标本进行Oct4表达的检测。结果在绝大多数(25/27)的肾细胞癌标本中有4．6%～5．5%的Oct4阳性细胞,RT-PCR显示10例肾细胞癌组织在相应位置均出现目的基因Oct4的条带,正常肾皮质组织和癌旁组织并没有出现Oct4条带。Oct4表达的阳性率及强度与肾细胞癌分期和分型无明显相关。结论肾细胞癌组织中有少量表达全能干细胞标志物Oct4的细胞,是否为肾癌干细胞有待进一步研究。
Objective To investigate the expression of Oct4, a key transcription factor of stem cells in renal cell carcinoma (NPC), and to explore the relationship between Oct4 expression and the type and stage of renal cell carcinoma. Methods The expression of Oct4 in 10 cases of renal cell carcinoma was detected by immunohistochemistry in 27 cases and reverse transcription - polymerase chain reaction (RT - PCR). Results In most of the renal cell carcinoma samples (25/27), there were 4.6% -5.5% of Oct4 positive cells. RT-PCR showed that all the 10 cases of renal cell carcinoma showed the target gene Oct4 Oct4 bands were not observed in normal renal cortical tissues and paracancerous tissues. The positive rate and intensity of Oct4 expression were not significantly correlated with the stage and type of renal cell carcinoma. Conclusions There is a small amount of cells expressing the pluripotent stem cell marker Oct4 in renal cell carcinoma tissues, and whether renal carcinoma stem cells need further study.