论文部分内容阅读
利用透射电子显微镜技术研究了七带石斑鱼(Epinephelusseptemfasciatus)精子的超微结构,结果发现:七带石斑鱼精子由头部和尾部(鞭毛)两部分组成,没有明显的中片,头部前段无顶体,主要有高电子密度的球形细胞核,核中主要是致密的染色质,还分布有一些核泡,核外由核膜包围,将核与细胞质分隔开,核膜最前端紧贴质膜,核后端有植入窝,近端中心粒和中心粒间体位于其中。头部后端为袖套结构,由质膜包围的袖套结构延伸到后部,使整个头部呈椭球形,质膜后端沿鞭毛形成一个袖套腔,袖套结构中分布有中心粒复合体、线粒体以及囊泡等。精子细胞的细胞膜不光滑,呈现小的皱折。七带石斑鱼精子的尾部细长,紧接于头部细胞核,穿过袖套腔,通过基体与核紧密结合,中心粒复合体也位于此,且近端中心粒和远端中心粒呈钝角,鞭毛的轴丝由此形成,基体与鞭毛间的过渡区轴丝无中央微管,呈“9+0”型。尾部的主要结构为轴丝,其轴丝为典型的“9+2”结构,其起始端开始于前端基体,轴丝外仅有少许细胞质。
Transmission electron microscopy was used to study the ultrastructure of spermatozoa in Epinephelus septemfasciatus. The results showed that the spermatozoa in the seven-band grouper consisted of two parts, the head and the tail (flagella) There is no acrosome in the anterior segment, mainly spherical nucleus with high electron density. The nucleus is mainly dense chromatin, and some nucleoli are distributed. The nucleus is surrounded by the nuclear membrane, separating the nucleus and the cytoplasm. The forefront of the nuclear membrane Paste the plasma membrane, the nuclear back end with implant nest, the proximal center of the central granulosa and intergranular body located in them. The back of the head is a cuff structure, the cuff structure surrounded by the plasma membrane extends to the back so that the entire head is ellipsoidal, and a cuff cavity is formed along the flagellum at the back end of the plasma membrane. Centrosomes are distributed in the cuff structure Complex, mitochondria and vesicles and so on. Sperm cell membrane is not smooth, showing small wrinkles. The tail of the seven-band grouper sperm is slender, immediately adjacent to the head nucleus, through the cuff cavity, tightly bound to the nucleus by the matrix, where the centromere complex is located, and the proximal and distal centromere Obtuse angle, the flagellum axis wire is formed, the transition zone between the matrix and flagella without central axis of microtubules, was “9 +0” type. The main structure of the tail axis of the shaft, the axis of the wire is a typical “9 +2 ” structure, starting from the beginning of the front end of the matrix, only a small amount of cytoplasm outside the axis of the shaft.