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目的建立高效毛细管电泳(HPCE)法同时测定蒙药小白蒿不同加工品中5种黄酮类成分的方法。方法小白蒿经不同加工工艺处理后,用HPCE法分别测定其中5种黄酮类成分,并对其进行比较。结果回归方程分别为5,7,3′-三羟基-4′-甲氧基黄酮Y=0.114 3 X+0.032 7,r=0.999 8;5,3′-二羟基-6,7,4′-三甲氧基黄酮Y=0.100 9 X+0.048 5,r=0.999 6;槲皮素Y=0.055 5 X+0.026 8,r=0.999 6;5,7,3′-三羟基-6,4′-二甲氧基黄酮Y=0.113 2 X-0.016 1,r=0.999 3;木犀草素Y=0.097 9 X-0.029 5,r=0.999 4。5种黄酮类成分分别在1.00～40.00、10.00～200.00、5.00～100.00、1.00～40.00、1.00～40.00μg/mL线性关系良好。平均回收率分别为98.44%、97.75%、97.73%、97.98%、98.07%;RSD分别为1.60%、1.03%、1.57%、0.94%、1.20%。结论不同加工工艺对小白蒿中5种黄酮类成分有一定影响;以5种黄酮类成分为分析指标,小白蒿最佳加工工艺为60℃加热干燥。
Objective To establish a high performance capillary electrophoresis (HPCE) method for the simultaneous determination of five flavonoids in different processed products of Mongolian medicine. Methods After treatment of Artemisia annua L. with different processing techniques, five flavonoids were determined by HPCE and compared with each other. Results The regression equations were: 5,7,3’-trihydroxy-4’-methoxyflavone Y = 0.114 3 X + 0.032 7, r = 0.999 8; 5,3’-dihydroxy-6,7,4 ’ - Trimethoxyflavone Y = 0.100 9 X + 0.048 5, r = 0.999 6; Quercetin Y = 0.055 5 X + 0.026 8, r = 0.999 6; 5,7,3’-trihydroxy-6,4 ’ - dimethoxyflavone Y = 0.113 2 X-0.016 1, r = 0.999 3; Luteolin Y = 0.097 9 X-0.029 5, r = 0.999 4.5 kinds of flavonoids were 1.00 ~ 40.00,10.00 ~ 200.00,5.00 ~ 100.00,1.00 ~ 40.00,1.00 ~ 40.00μg / mL linear relationship is good. The average recoveries were 98.44%, 97.75%, 97.73%, 97.98% and 98.07%, respectively. The RSDs were 1.60%, 1.03%, 1.57%, 0.94% and 1.20%, respectively. Conclusion Different processing techniques have certain influence on the five flavonoids in Artemisia annua L. The best processing technology of Artemisia annua is heating and drying at 60 ℃ with the analysis of five flavonoids.