目的:研究联合应用硫化砷和α干扰素是否可以增强对K562细胞的作用。方法:采用PCR-ELISA法测定端粒酶活性;流式细胞仪分析细胞凋亡。IFN-α和硫化砷的终浓度分别均为10000U/mL和0.6mg/L。结果:单独应用IFN-α或硫化砷8d,可以诱导37.8%和37%的K562细胞出现凋亡;同时应用IFN-α和硫化砷8d或先单独应用IFN-α3d,再单独应用硫化砷5d,K562细胞的凋亡率分别为59.9%和60.37%;端粒酶活性的抑制,单用硫化砷为71.3%,联合用药分别为81.2%和78.8%。结论:联合应用IFN-α和硫化砷较单独用药相比,可以显著提高K562细胞的凋亡,抑制细胞端粒酶活性。提示IFN-α可能促进硫化砷诱导K562细胞凋亡。 OBJECTIVE: To investigate whether the combination of arsenic sulfide and alpha interferon can enhance the effect on K562 cells. Methods: The telomerase activity was determined by PCR-ELISA. Apoptosis was analyzed by flow cytometry. The final concentrations of IFN-α and arsenic sulfide were 10000 U / mL and 0.6 mg / L, respectively. Results: Apoptosis was induced in 37.8% and 37% of K562 cells treated with IFN-α or arsenic sulfide alone for 8 days. IFN-α and arsenic sulfide were also used for 8 days or IFN-α3d alone. The apoptotic rates of K562 cells were 59.9% and 60.37%, respectively. The inhibition of telomerase activity was 71.3% for arsenic sulfide alone and 81.2% and 78.8% for the combined drugs respectively. Conclusion: The combined application of IFN-α and arsenic sulfide can significantly increase the apoptosis of K562 cells and inhibit the telomerase activity compared with the drug alone. It is suggested that IFN-α may promote arsenic sulfide-induced K562 cell apoptosis.