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目的探讨肾移植受者的某些细胞因子及其受体基因多态性与术后感染的相关性。方法采用自行研制的细胞因子及其受体单核苷酸多态性检测芯片,分析129例肾移植受者的肿瘤坏死因子(TNF-α)、白细胞介素10(IL-10)、转化生长因子(TGF-β1)、IL-4、IL-6及其受体的21个位点的基因多态性分布情况,并按照患者术后是否发生感染进行分组比较。结果5种细胞因子及其受体单核苷酸多态性在感染组和非感染组中分布明显不同,分别是基因型IL-6R(-183GG、G/A)、IL-10(-824C/T,-597C/A)及TNF-α(-308GG、G/A);等位基因为IL-10R1(1112G/A)、IL-6R(-183G/A)、IL-4R(1902A/G)、TNF-α(-308G/A)及TGF-β1(+869T/C)。结论基因型IL-6R(-183GG)、IL-10(-824C/T,-597C/A)及TNF-α(-308GG),等位基因IL-4R(1902A)、IL-6R(-183G)、IL-10R1(1112G)、TNF-α(-308G)及TGF-β1(+869C)是肾移植后感染的易患因素;而基因型IL-6R(-183G/A)和TNF-α(-308G/A)可能为移植后感染的非易患因素。
Objective To investigate the relationship between some cytokines and their receptor gene polymorphisms and postoperative infection in renal transplant recipients. Methods A total of 129 renal allograft recipients were enrolled in this study. Tumor necrosis factor (TNF-α), interleukin-10 (IL-10) (TGF-β1), IL-4, IL-6 and their receptors in 21 loci gene polymorphism distribution, and in accordance with whether patients with postoperative infection grouping comparison. Results The distributions of five cytokines and their receptor SNPs were significantly different among infected and noninfected groups, which were genotype IL-6R (-183GG, G / A), IL-10 (-824C / T, -597C / A) and TNF-α (-308GG, G / A). The alleles were IL-10R1 (1112G / A), IL- G), TNF-α (-308G / A) and TGF-β1 (+ 869T / C). Conclusion The genotypes of IL-6R (-183GG), IL-10 (-824C / T, -597C / A) and TNF-α (-308GG) ), IL-10R1 (1112G), TNF-α (-308G) and TGF-β1 (+ 869C) are risk factors for post-renal allograft infection; genotype IL-6R (-308G / A) may be a non-predisposing factor for post-transplant infection.