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目的探讨中药复方水煎液Ⅰ、Ⅱ、Ⅲ号对大鼠海马神经细胞缺氧损伤有无保护作用。方法 1 6只Wistar大鼠 ,随机分为 4组 ,第 1组每天分两次灌喂生理盐水 4ml,第 2— 4组分别每天分两次灌喂等量中药复方水煎液Ⅰ、Ⅱ、Ⅲ号 ,3天后取血 ,制备血清。另取新生 48小时内的Wistar大鼠 ,在无菌条件下断头取脑 ,分离海马 ,制成细胞悬液体外培养。在培养第 7天将培养皿随机分为 5组 ,第 1组为正常对照组 ,第 2— 5组分别加入灌喂生理盐水及中药复方Ⅰ、Ⅱ、Ⅲ号的大鼠血清 ,2 4小时后 2— 5组培养皿置缺氧环境 1小时 ,之后再培养 2 4小时 ,检测各培养皿细胞存活率及培养液中乳酸脱氢酶 (LDH)活性和丙二醛 (MDA)含量。结果灌喂复方Ⅰ号组和Ⅱ号组大鼠血清的培养液中LDH的活性和MDA的含量明显低于缺氧对照组 ,细胞存活率高于缺氧对照组 ,均有统计学意义 (P <0 .0 5— 0 .0 0 1 )。结论中药复方Ⅰ号和Ⅱ号对体外培养大鼠海马神经元缺氧损伤具有保护作用。
Objective To investigate whether Chinese herbal decoctions I, II, and III have protective effects on rat hippocampal neuronal hypoxic injury. Methods Sixty-six Wistar rats were randomly divided into 4 groups. The first group was given 4 ml of physiological saline twice a day, and the second group was fed twice a day with the same amount of Chinese compound compound decoction I and II. On the third day, blood was taken 3 days later to prepare serum. Wistar rats within 48 hours of neonatal recovery were also decapitated and brains were removed under aseptic conditions. The hippocampus was isolated and cultured as a cell suspension. On the 7th day of culture, the culture dishes were randomly divided into 5 groups. The first group was normal control group, and the second to fifth groups were fed with rat serum fed with physiological saline and Chinese herbal compound I, II, and III, respectively, for 24 hours. After 2 to 5 sets of culture dishes were placed in anoxic environment for 1 hour, and then cultured for 24 hours. The viability of cells and the activity of lactate dehydrogenase (LDH) and malondialdehyde (MDA) in the culture medium were measured. Results The activity of LDH and the content of MDA in the serum of rats fed with Compound I and II groups were significantly lower than that of the hypoxic control group, and the cell survival rate was higher than that of the hypoxic control group (P<0.05). <0 .0 5-0. 0 0 1 ). Conclusion Chinese Herbal Compound No. I and II have a protective effect on rat hippocampal neurons from hypoxia injury in vitro.