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目的 探讨熊去氧胆酸 (ursodeoxycholicacid ,UDCA)的应用对胆汁淤积症 (ICP)孕鼠脏层卵黄囊细胞膜脂质流动性及胎盘组织谷光甘肽 (glutathione,GSH)含量的影响。 方法 选择妊娠SD大鼠 6 0只 ,随机分成三组 ,每组 2 0只 :(1)自妊娠第 13天起对照组孕鼠皮下注射精制植物油 2 .5ml/ (kg·d) ,ICP非治疗组和治疗组分别皮下注射孕酮 75mg/ (kg·d)和 17α 乙炔雌二醇1.2 5mg/ (kg·d) ,直至孕 17d。 (2 )自妊娠第 17天起 ,对照组、ICP非治疗组孕鼠行生理盐水 5ml/ (kg·d)灌胃 ,治疗组孕鼠UDCA 5 0mg/ (kg·d)灌胃。 (3)三组孕鼠均于妊娠第 2 1天处死 ,记录死胎数并计算死胎率 ,取出子宫 ,仔细分离胚胎脏层卵黄囊膜 ,制备脏层卵黄囊细胞悬液 ,应用 5 ,5’ 二硫代双(二硝基苯甲酸 )直接法测定GSH的含量 ;应用 1,6 二苯已三烯荧光标记法测定细胞膜脂质流动性。 结果 (1)孕鼠胚胎脏层卵黄囊细胞膜脂质流动性及胎盘组织GSH含量 :ICP非治疗组GSH含量、偏振度P值分别为 :(1.12± 0 .0 2 )mmol/gprot、0 .32 2± 0 .0 0 2 ;对照组分别为 :(1.5 6±0 .0 7)mmol/ gprot、0 .2 81± 0 .0 0 3;治疗组分别为 :(1.38± 0 .0 3)mmol/gprot,0 .171± 0 .0 0 3。ICP非治疗组与对照组相比 ,GSH含量明显下降 (P <0 .0 5
Objective To investigate the effects of ursodeoxycholic acid (UDCA) on the lipid fluidity and the content of glutathione (GSH) in the visceral yolk sac of the pregnant rat with cholestasis (ICP). Methods Sixty pregnant SD rats were randomly divided into three groups (n = 20): (1) Control pregnant rats were subcutaneously injected with 2.5ml / (kg · d) The treatment group and the treatment group were subcutaneously injected progesterone 75mg / (kg · d) and 17α ethinyl estradiol 1.2 5mg / (kg · d), respectively, until the pregnant 17d. (2) From the 17th day of gestation, the pregnant rats in the control group and non-ICP treatment group were given 5ml / (kg · d) saline intragastrically. The pregnant rats in the treatment group were fed with 50mg / (kg · d) UDCA. (3) The three groups of pregnant rats were sacrificed on the 21st day of pregnancy, the number of stillbirths was recorded and the stillbirth rate was calculated. The uterus was removed and the yolk sac membrane of the embryonic visceral layers was carefully isolated to prepare the visceral layer yolk sac cells suspension. Dithiobis (dinitrobenzoic acid) direct method for the determination of GSH content; application of 1,6-diphenyltetraene fluorescence labeling method for measuring cell membrane lipid fluidity. Results (1) The lipid fluidity and the content of GSH in placental yolk sac of pregnant mice embryos: The content of GSH and the polarization P value of ICP non-treatment group were (1.12 ± 0.02) mmol / gprot, respectively. 32 2 ± 0. 0 0 2 respectively; the control group was (1.5 6 ± 0. 0 7) mmol / gprot and 0 2 81 ± 0 0 0 3 respectively; the treatment group was (1.38 ± 0. ) mmol / gprot, 0 .171 ± 0 .0 0 3. Compared with the control group, the GSH level in ICP non-treated group decreased significantly (P <0.05)