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目的探讨木天蓼提取物对人表皮角质形成细胞(HEK)产生血管内皮生长因子(VEGF)的影响及木天蓼提取物对HEK增殖作用的影响。方法采用不同浓度、不同种类的木天蓼提取液分别对HEK进行刺激,并在不同时间回收培养液,采用MTT法测定不同时间、不同刺激物作用下HEK增殖活力的变化;采用ELISA技术检测回收的培养液中VEGF的浓度。结果不同浓度、不同种类的木天蓼提取物对HEK的增殖活力均无显著影响(P=0.44);木天蓼的醇溶性提取物(APE)及水溶性提取物(APW)对HEK分泌VEGF均有抑制作用(APE:P<0.01;APW:P=0.02);就抑制HEK分泌VEGF的作用而言,APE作用明显强于APW,差异有统计学意义(P=0.02);APE对HEK分泌VEGF的抑制作用具有浓度及时间依赖性。结论木天蓼提取液能明显抑制HEK分泌VEGF,但对HEK的增殖活力无明显的影响。本研究为研发减轻VEGF引起的红斑的药物提供了一定的理论依据。
Objective To investigate the effects of Polygonum multiflorum extract on vascular endothelial growth factor (VEGF) production by human epidermal keratinocytes (HEK) and the effects of Polygonum multiflorum extract on HEK proliferation. Methods HEK was stimulated by different concentrations and different kinds of Polygonum multiflorum extract, and the culture fluid was recovered at different time. The proliferation of HEK cells was measured by MTT assay at different time and different stimuli. HEK-293 cells were harvested by ELISA. Of the culture medium VEGF concentration. Results Different concentrations and different kinds of Polygonum multiflorum extract had no significant effect on HEK proliferation (P = 0.44). Alcohol-soluble extract (APE) and water-soluble extract (APW) (APE: P <0.01; APW: P = 0.02). The effect of APE on the secretion of VEGF by HEK was significantly stronger than APW (P = 0.02) The inhibitory effect of VEGF is concentration-dependent and time-dependent. Conclusion Polygonum multiflorum extract can significantly inhibit the secretion of VEGF by HEK, but has no obvious effect on the proliferation activity of HEK. This study provides a theoretical basis for the development of drugs to reduce the erythema caused by VEGF.