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目的 :探讨 BL U基因在 T/ NK细胞淋巴瘤发生中的作用与分子病理诊断中的应用价值。方法 :选用已经确诊为鼻 T/ NK细胞淋巴瘤 1 2例的冻存组织标本与 2 0例石蜡组织标本 ,从组织中提取 DNA,用 MSP方法检测 BL U基因启动子的甲基化状态。结果 :在 1 2个冰冻组织病例中 ,用 M引物扩增的产物 ,BLU基因全部为阳性 ,U引物扩增的产物 ,BL U基因 1 1例为阳性 ;2 0例石蜡组织病例中 ,M引物扩增的产物 6例阳性 ,U引物扩增的产物 1 2例阳性。表明 BLU基因在鼻 T/ NK细胞淋巴瘤中有高度甲基化 ,冰冻组织中的甲基化检出率明显高于石蜡组织。结论 :BL U基因启动子 Cp G岛在鼻 T/ NK细胞淋巴瘤中普遍高度甲基化 ,表明此抑癌基因被失活 ,可能是 T/ NK细胞淋巴瘤的发病机制之一。
Objective: To investigate the role of BL U gene in the pathogenesis of T / NK cell lymphoma and its molecular diagnostic value. Methods: Frozen tissue specimens and 20 paraffin tissue specimens from 12 cases of nasal T / NK cell lymphomas were selected. DNA was extracted from tissues and the methylation status of BL U gene promoter was detected by MSP method. RESULTS: Among the 12 frozen tissue cases, all of the products amplified by M primer and BLU gene were positive. The products amplified by U primer and BL U gene were positive in 11 cases. Among 20 cases of paraffin wax tissue samples, M Six of the products amplified by the primer were positive, and 12 products amplified by the U primer were positive. The results showed that the BLU gene was highly methylated in nasal T / NK cell lymphoma, and the methylation detection rate in frozen tissue was significantly higher than that in paraffin tissue. CONCLUSION: CpG island of BL U promoter is generally highly methylated in nasal T / NK cell lymphoma, indicating that inactivation of this tumor suppressor gene may be one of the pathogenesis of T / NK cell lymphoma.