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目的 探讨慢性阻塞性肺疾病（COPD）对大鼠膈神经放电和呼吸运动的影响。方法 随机将60只Wistar大鼠分为两组，每组30只。实验组：熏香联合气管内滴注猪胰蛋白酶建立大鼠COPD模型；对照组：不给予熏香，d15气管内滴注生理盐水20 u／100 g。分别记录每组大鼠膈神经放电持续时间及放电幅度和间期，并进行对比。结果 实验组大鼠膈神经的放电幅度较对照组明显增强，差异有统计学的意义（P ＜0．05）；两组的膈神经持续放电时间、放电间期及放电面积比较无明显统计学差异（ P ＞0．05）；两组吸气相时间和膈神经放电活动均呈正相关。结论 COPD可影响大鼠膈神经放电和呼吸运动，使膈神经放电活动增强，出现呼吸中枢吸气驱动增高的现象，提示大鼠呼吸中枢的活动可能发生了改变。“,”Objective] To explore the effect of chronic obstructive pulmonary disease(COPD) on phrenic nerve discharge and respiratory movement in rat .[Methods]Totally 60 Wistar rats were randomly divided into two groups with 30 in each group .In experimental group ,COPD model was established by incense combined with porcine trypsin endotracheal instillation .In control group ,incense was not given ,but normal saline 20u/100g was instilled in trachea on d15 .The duration ,amplitude and interval of rats in each group were recorded and compared .[Results]Compared with control group ,the amplitude of phrenic nerve discharge in experimen‐tal group was increased ,and there was significant difference( P 0 .05) .Inspiratory phase time was positively correlated with phrenic nerve discharge activity in two groups .[Conclusion] COPD can influence of phrenic nerve discharge and respiratory movement ,enhance phrenic nerve discharge activity in rat and has the phenomenon such as the increasing of inspiration driving in respiratory center ,which indicates that the activity of respiratory center in rat may be changed .