小分子是通过蛋白生物活性而被认识的。因此,合理的药物设计的第一步应该是阐明配体及其受体之间的分子相互作用。基于近来重组技术的发展,在接受界面的配体方面,生物功能机制的研究已经成为一种挑战和重要的化学课题。通常用于研究受体功能部位的三个主要的研究有它们自己的优势和局限性。光谱法能够分析原子水平上的配体-受体的相互作用,但这些研究通常需要一定量的稳定的纯蛋 Small molecules are recognized by their biological activity. Therefore, the first step in rational drug design should be to elucidate the molecular interactions between ligands and their receptors. Based on recent advances in recombinant technology, the study of biological functional mechanisms has become a challenge and an important chemical topic in accepting ligands at the interface. The three main studies commonly used to study functional areas of receptors have their own advantages and limitations. Spectroscopy enables the analysis of ligand-receptor interactions at the atomic level, but these studies usually require a certain amount of stabilized pure egg