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目的观察肺孢子菌肺炎(PCP)大鼠肺泡巨噬细胞(AM)吞噬功能及支气管肺泡灌洗液(BALF)、AM培养上清TNF-α、IL-6的变化。方法 Wistar大鼠连续肌肉注射地塞米松建立免疫抑制PCP模型,检测大鼠AM吞噬功能及BALF、AM培养上清TNF-α、IL?6水平,同时设正常对照组。结果 PCP模型组大鼠AM吞噬鸡红细胞百分率为(20.61±2.04)%,吞噬指数为0.25±0.21,均明显低于对照组的(25.45±3.1)%和0.31±0.16(P均<0.05)。BALF中TNF?α为(16.84±0.86)pg/ml,IL?6为(1.05±0.19)pg/ml,均明显高于对照组的(12.48±0.84)pg/ml和(0.86±0.11)pg/ml(P均<0.05);AM培养上清中TNF?α、IL?6差异均无统计学意义(P均>0.05)。结论使用糖皮质激素造成大鼠免疫抑制诱发PCP后,大鼠AM吞噬功能降低,BALF中TNF-α、IL-6水平升高。
Objective To observe the phagocytosis of alveolar macrophages (AM) and the changes of TNF-α and IL-6 in bronchoalveolar lavage fluid (BALF) and AM supernatant of Pneumocystis pneumoniae (PCP) rats. Methods Wistar rats were injected intramuscularly with dexamethasone to establish immunosuppressive PCP model. AM phagocytosis function and TNF-α, IL-6 levels in BALF and AM culture supernatants were detected. At the same time, normal control group was established. Results The percentage of erythrocytes of AM phagocytosis chicken in PCP model group was (20.61 ± 2.04)% and the phagocytic index was 0.25 ± 0.21, which were significantly lower than that in control group (25.45 ± 3.1)% vs 0.31 ± 0.16 (P <0.05). TNFα in BALF was (16.84 ± 0.86) pg / ml and IL 6 was (1.05 ± 0.19) pg / ml in BALF, which were significantly higher than those in control group (12.48 ± 0.84 pg / ml and 0.86 ± 0.11 pg / ml (P <0.05). There was no significant difference in the levels of TNF-α and IL-6 in AM culture supernatant (all P> 0.05). Conclusions The use of glucocorticoid induced PCP induced by immunosuppression in rats decreased phagocytosis of AM and increased the levels of TNF-α and IL-6 in BALF.