论文部分内容阅读
目的:建立三七和三七支根的固体三维荧光指纹图谱。方法:以血塞通冻干粉作为对照,采用固体三维荧光技术建立三七及三七支根的固体荧光指纹图谱。结果:血塞通冻干粉有230 nm/330 nm、280 nm/660 nm、300nm/340 nm、370 nm/590 nm、440 nm/550 nm、490 nm/610 nm的荧光峰,最强的为300 nm/340 nm的荧光峰。三七粉末具有230 nm/340 nm、290 nm/340 nm、370 nm/480 nm、430 nm/540 nm的荧光峰,最强为290 nm/340 nm的荧光峰;三七支根的荧光峰在280 nm/450 nm、290 nm/350 nm、350 nm/440 nm,最强为350 nm/440 nm的荧光峰。三者均有290~300 nm/340~350 nm的荧光峰,且血塞通冻干粉和三七均是该峰最强,而三七支根是350 nm/440 nm的荧光峰最强。三七和血塞通冻干粉还有230 nm/330~340 nm的荧光峰,三七支根没有。结论:通过该方法获得了相应样品的固体三维荧光光谱图。在建立的固体三维荧光光谱中选取以上两个荧光峰作为特征荧光峰进行检测,可以高效快速的鉴别三七和三七支根。
Objective: To establish a solid three-dimensional fluorescent fingerprint of the roots of Panax notoginseng and Panax notoginseng. Methods: Xuesaitong freeze-dried powder as a control, the solid three-dimensional fluorescence technology to establish three seven and thirty-seven roots of the solid fluorescent fingerprint. Results: Xuesaitong freeze-dried powder has the fluorescence peaks of 230 nm / 330 nm, 280 nm / 660 nm, 300 nm / 340 nm, 370 nm / 590 nm, 440 nm / 550 nm, 490 nm / Fluorescence peak at 300 nm / 340 nm. The notoginseng powder has the fluorescence peak at 230 nm / 340 nm, 290 nm / 340 nm, 370 nm / 480 nm and 430 nm / 540 nm, and strongest at 290 nm / 340 nm. The fluorescence peak Fluorescence peak at 280 nm / 450 nm, 290 nm / 350 nm, 350 nm / 440 nm, and strongest at 350 nm / 440 nm. The fluorescence peaks of 290-300 nm / 340-350 nm were observed in all three samples, and the peaks of Xuesaitong freeze-dried powder and Panax notoginseng were the strongest, while those of Panax notoginseng was the strongest at 350 nm / 440 nm . Panax notoginseng freeze-dried powder and 230 nm / 330 ~ 340 nm fluorescence peak, not the three seven roots. Conclusion: The solid three-dimensional fluorescence spectra of the corresponding samples were obtained by this method. In the established three-dimensional solid-state fluorescence spectra of the above two fluorescence peaks as the characteristic fluorescence peak detection, rapid and effective identification of notoginseng and thirty-seven roots.