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A high-performance capillary electrophoresis with amperometry detection method(CE-AD) has been developed for the analysis of flavonoids and anthraquinones(emodin,kaempferol,apigenin,luteolin and rhein) in chrysanthemum.Under optimum conditions,these five analytes were base-line separated within 17 min using a borate-phosphate running buffer(1.5×10~(-2) mol/L borate-3×10~(-2) mol/L phosphate running buffer,pH 9.0) at a working potential of +0.90 V(vs.SCE) and a separation voltage of 19 kV.The linear relationship between concentration and current response was obtained with detection limits(S/N = 3) ranging from 1.0×10~(-7) to 2.1×10~(-7) g/mL for all analytes.This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures,the assay results were satisfactory.
A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum.Under optimum conditions, these five analytes were base-line separated within 17 min using a borate-phosphate running buffer (1.5 × 10 -2 mol / L borate-3 × 10 -2 mol / L phosphate running buffer, pH 9.0) at a working potential of +0.90 V (vs.SCE) and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits (S / N = 3) ranging from 1.0 × 10 -7 to 2.1 × 10 ~ ( -7) g / mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.