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目的探讨精胺对放射损伤的保护机制及可能的治疗效果。方法昆明种雄性小鼠随机分为对照组（Ａ组），单放组（Ｂ组），放射前注入精胺组（Ｃ组），放射后注入精胺组（Ｄ组）；６Ｇｙ一次性全身~（６０）Ｃｏｒ射线照射后，在１５ｄ内观察各组小鼠体重、存活率、以及外周血和骨髓细胞数量的变化；用ＥＬＩＳＡ和放免的方法分别检测血清ＴＧＦ－β_１和ＴＮＦ－α的含量；用免疫组化法检测骨髓组织ＴＮＦ－α和ＴＧＦ－β_１的表达。结果①Ｃ、Ｄ两组小鼠的体重明显高于Ｂ组，存活率显著提高（ Ｐ＜０．０１）；②Ｃ、Ｄ两组血清及骨髓组织中ＴＮＦ－α和ＴＧＦ－β_１水平较Ｂ组显著降低。结论精胺对放射损伤的保护作用与其抑制造血抑制因子表达有关。
Objective To explore the protective mechanism of spermine on radiation injury and its possible therapeutic effect. Methods Kunming male mice were randomly divided into control group (group A), single group (group B), spermine group (group C) before radiation and spermine group (group D) after radiation. The changes of body weight, survival rate, number of peripheral blood and myeloid cells in each group were observed after ~ (60) Cor radiation. The levels of serum TGF-β 1 and TNF-α were detected by ELISA and radioimmunoassay Immunohistochemistry was used to detect the expression of TNF-αand TGF-β_1 in bone marrow. Results ① The body weight of mice in C and D groups was significantly higher than that of B group (P <0.01). ② The levels of TNF-α and TGF-β 1 in serum and bone marrow of C and D groups were significantly higher than those in B group reduce. Conclusion The protective effect of spermine on radiation injury is related to the inhibition of hematopoietic suppressor expression.