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目的:观察7种抗肿瘤药物对体外原代培养的胶质瘤细胞的作用,为临床化疗方案的设计提供参考。方法:32例术后病理证实为胶质瘤的新鲜标本取材,制备肿瘤单细胞悬液进行体外原代培养,与肿瘤药DDP,VM-26,VP-16,BCNU,VCR,PCB,Taxol的临床血浆峰值浓度(PPC)作用72小时,MTT法检测不同肿瘤个体对不同化疗药物的敏感和耐药情况。结果:成功进行了32例临床标本的原代培养及药敏试验,其PPC下的抑制率(IR%)>50%者,DDP有14例;VCR有5例;VM-26有7例;VP-16有9例;PCB有3例;BCNU有2例;Taxol有1例;其敏感性依次为:DDP>VP-16>VM-26>VCR>PBZ>BCNU>Taxol。结论:7种常用的抗肿瘤药物均有耐药的情况,进行化疗药物的敏感测定可以避免耐药药物的使用,为提高临床化疗效果提供指导。
Objective: To observe the effect of seven anti-tumor drugs on primary cultured glioma cells in vitro and provide references for the design of clinical chemotherapy regimens. Methods: Thirty-two fresh specimens of glioma were obtained from the pathological examination. Single cell suspension of the tumor was prepared for primary culture in vitro and incubated with tumor drugs DDP, VM-26, VP-16, BCNU, VCR, PCB, Taxol Clinical peak plasma concentration (PPC) effect of 72 hours, MTT assay of different tumor individual sensitivity to different chemotherapeutic drugs and drug resistance. Results: The primary culture and drug susceptibility tests of 32 clinical samples were successfully performed. The rate of IR% under PPC was> 50%, there were 14 cases of DDP, 5 cases of VCR, 7 cases of VM-26, VP-16 in 9 cases; PCB in 3 cases; BCNU in 2 cases; Taxol in 1 case; the sensitivity of the order: DDP> VP-16> VM- 26> VCR> PBZ> BCNU> Taxol. CONCLUSION: All 7 kinds of commonly used antineoplastic drugs are resistant. The sensitive determination of chemotherapeutic drugs can avoid the use of drug-resistant drugs and provide guidance for improving the effect of clinical chemotherapy.