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目的 检测 1194例初诊怀疑患强直性脊柱炎 (AS)和其它关节性疾病患者的HLA B2 7、B7、B13和B40位点抗原的分布 ,并分析这些位点的相互杂合和其抗原的交叉反应性。方法 微量细胞毒反应法 ,在同一块反应盘上同时检测。结果 1.在 1194例患者中 ,HLA B2 7抗原阳性率达38 6 % ;B7的抗原阳性率为 7 4% ;B13为 14 6 % ;B40为 13 4%。2 .在HLA B2 7阳性人群中 ,B7抗原的阳性率为 14 3% ,明显升高 ,而在HLA B2 7阴性人群中 ,B7的阳性率仅为 3 2 % ,明显降低 ;3.在44 7例HLA B2 7阳性人群中 ,未检测到HLA B2 7、B7、B13三种抗原皆为阳性者 ,说明B7和B13之间没有交叉 ;4.据此 ,在HLA B2 7阴性人群中 ,查到的HLA B7/B13阳性率 0 5 4%应代表这两个位点的杂合 ;5 .无论在HLA B2 7阳性还是阴性人群中 ,HLA B13/B40的抗原阳性率远高于HLA B7/B40的阳性率 ,说明B13和B40位点的杂合或交叉 ,远大于B7与B40之间的杂合或交叉。结论 检测HLA B2 7及其相关B位点抗原的频度有助于分析这些位点的杂合及其抗原的交叉反应性的特点
Objective To detect the distribution of antigens at HLA B2 7, B7, B13 and B40 loci in 1194 newly diagnosed patients with ankylosing spondylitis (AS) and other joint diseases, and to analyze the cross heterozygosity and antigen cross of these loci Reactivity. Methods Micro-cytotoxic reaction method, simultaneous detection of the same reaction plate. Results 1. Among 1194 patients, the positive rate of HLA B2 7 antigen was 38 6%; the positive rate of B7 antigen was 74%; B13 14.6%; and B40 13 4%. The positive rate of B7 antigen was 14.3% in HLA B2 7 positive population, but it was significantly higher in HLA B2 7 negative population, while the positive rate of B7 was only 32% Of the 7 HLA B2 7 positive individuals, none of the three antigens of HLA B2 7, B7, and B13 were detected as positive, indicating no cross between B7 and B13; 4. Accordingly, among HLA B2 7 negative individuals, The positive rate of HLA B7 / B13 to HLA-B7 / B13 should represent the heterozygosity of the two loci. 5. The positive rate of HLA B13 / B40 antigen in HLA B2 7 positive or negative groups is much higher than that of HLA B7 / The positive rate of B40 indicates that the B13 and B40 sites are heterozygous or crossed, which is much larger than that between B7 and B40. Conclusion The frequency of detection of HLA B2 7 and its associated B-site antigens is helpful in analyzing the heterozygosity and cross-reactivity of their antigens at these sites