【摘 要】
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Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondrial cDNA of tuber mustard CMS line. T1243 was a transcript with an intron unspliced
【机 构】
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College of Life Science and Technology,College of Agriculture and Biotechnology,Key Biotechnology La
论文部分内容阅读
Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondrial cDNA of tuber mustard CMS line. T1243 was a transcript with an intron unspliced, which has the basic characteristics of type Ⅱ intron. The expressions of the two transcripts were analyzed by reverse transcription PCR (RT-PCR). The results showed that, at seedling stage, the expression of T gene was mainly in the form of T1170 but decreased with the development gradually, while the expression abundance of another transcript, T1243, increased gradually. The T1243 was prevalent at the profuse flowering stage. The expression pattern was confirmed by Northern blot analysis. These results suggested that the alternative spliced mitochondrial T gene transcripts were related to CMS of tuber mustard.
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