在抵御入侵的微生物时,多形核白细胞和单核吞噬细胞释出一些反应性氧中间体(ROI),如过氧基(O_2~-)、过氧化氢(H_2O_2~-)和羟基(OH~-)等,它们起着一种重要的非特异性免疫效应作用。恶性疟原虫抗原能激活吞噬细胞产生ROI。 吞噬细胞经蛋白激酶C激活剂PMA(卟啉醇肉豆寇酸乙酸酯)、趋化性肽FMLP(甲酰基甲硫氨酰基苯丙氨酸)以及细胞因子TNF(肿瘤坏死因子)刺激后,能产生ROI;加入化学发光剂,如鲁米诺后可发光,而被定量检测。 In defense against invading microorganisms, polymorphonuclear leukocytes and mononuclear phagocytes release reactive oxygen intermediates (ROIs) such as O 2 -, H 2 O 2, and OH groups ~ -), etc., they play an important role in non-specific immune effects. Plasmodium falciparum antigens activate phagocytes to produce ROI. Phagocytic cells were stimulated by protein kinase C activators PMA (porphyrin myristate acetate), chemotactic peptide FMLP (formylmethionyl phenylalanine), and cytokine TNF (tumor necrosis factor) , Can produce ROI; adding chemiluminescents, such as luminol can be luminous, and was quantitatively detected.