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目的:研究不同脱色方法对全真一气汤多糖含量的影响,选择适宜的脱色方法,并初步探讨其对免疫抑制小鼠外周血免疫功能的影响。方法:分别采用大孔树脂静态吸附法、活性炭吸附法、过氧化氢氧化法进行脱色,以色素脱除率和多糖含量为指标,进行脱色效果比较;采用环磷酰胺造成免疫抑制模型,观察全真一气汤多糖对小鼠外周血白细胞、淋巴细胞及IL-2的影响。结果:大孔树脂静态吸附法处理后,多糖的色素脱除率为(48.32±1.05)%,多糖含量(39.63±1.08)%,相比粗多糖,含量有所提高。活性炭和过氧化氢处理后,色素脱除率虽然分别达到(68.49±1.02)%,(68.98±0.69)%,但多糖含量降低;全真一气汤多糖可对抗免疫抑外周血白细胞及淋巴细胞数目的降低,刺激外周血血清IL-2分泌的增加。结论:大孔树脂法适宜用于全真一气汤多糖脱色素;全真一气汤多糖可以提高免疫抑制小鼠的免疫功能。
OBJECTIVE: To study the effect of different decolorization methods on the polysaccharide content of Quanzhengyiqi decoction, and to select the suitable decolorization method and to explore its influence on immune function of immunosuppressed mice. Methods: The macroporous resin was used for static adsorption, activated carbon adsorption and hydrogen peroxide oxidation respectively, and the decolorization effect was compared with the removal rates of pigment and polysaccharide. The immunosuppressive model was induced by cyclophosphamide and observed Effects of Zhenyi Qi Tang polysaccharide on leucocytes, lymphocytes and IL-2 in peripheral blood of mice. Results: After macroporous resin was adsorbed by static method, the pigment removal rate was (48.32 ± 1.05)% and polysaccharide content was (39.63 ± 1.08)%, which was higher than that of crude polysaccharide. After the activated carbon and hydrogen peroxide treatment, although the pigment removal rate reached (68.49 ± 1.02)% and (68.98 ± 0.69)%, respectively, the polysaccharide content decreased. Quanzhengyiqi polysaccharide could counteract the number of immunosuppressive peripheral blood leukocytes and lymphocytes Decreased, stimulated the increase of peripheral blood serum IL-2 secretion. Conclusion: The macroporous resin method is suitable for Quanzhengyiqi Decoction. Quanzhengyiqi Tang polysaccharide can improve immunosuppression in immunosuppressed mice.