生药乙醇提取液经聚酰胺柱净化,在硅胶G薄层上,环己烷—乙酸乙酯(1:1)为展开剂,分离了马桑毒素(A)、羟基马桑毒素(B)、马桑亭(C)及另一未知内酯(D),用香草醛—硫酸的乙醇溶液显色后,薄层光密度法测定。本法显色灵敏、稳定,A,B,C在生药中的回收率分别为96.3±5.1,97.7±4.7和97.4±4.6％。用本法测定了马桑寄生叶、马桑子及其注射液以及马桑叶中所含以上内酯成分。 The crude drug ethanol extract was purified by a polyamide column. On a thin layer of silica gel G, cyclohexane-ethyl acetate (1:1) was used as a developing agent to separate the sarcotoxin (A) and hydroxymasonox toxin (B). Masoning (C) and another unknown lactone (D) were visualized by thin layer densitometry after developing with vanillin-sulfuric acid in ethanol. This method is sensitive and stable, and the recoveries of A, B and C in crude drugs are 96.3±5.1, 97.7±4.7 and 97.4±4.6%, respectively. This method was used to determine the contents of the above lactones in the Marsdenia parasites, Malus sieci and their injections, and in the leaves of Magnesia.