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目的建立离子色谱法测定丁二磺酸腺苷蛋氨酸中丁二磺酸离子的含量。方法采用岛津LC-10AT高压泵、ALLTECHM650电导检测器、ALLTECH M641抑制器,Dionex AS4A-SC色谱柱,8mmol/LNa2CO3/1mmol/LNaHCO3溶液为流动相,流速1ml/min。结果本方法的最低检测限和最低定量限分别为0.04μg/ml和0.12μg/ml;丁二磺酸含量在0.25～8μg/ml范围内与其峰面积呈良好的线性关系,r=0.999(n=7);峰面积/称样量的RSD=0.31%(n=6),平均回收率为100.10%,RSD=1.28%(n=9)。结论以上数据证明所选择的色谱柱、离子色谱条件及测定方法用于测定丁二磺酸腺苷蛋氨酸中丁二磺酸含量灵敏度高、准确可靠、简单快速。
Objective To establish an ion chromatography method for the determination of butane disulfonate ion in butanedioic acid methionine. Methods Shimadzu LC-10AT high pressure pump, ALLTECHM650 conductivity detector, ALLTECH M641 suppressor, Dionex AS4A-SC column, 8mmol / LNa2CO3 / 1mmol / LNaHCO3 solution as the mobile phase, flow rate of 1ml / min. Results The minimum detection limit and the minimum limit of quantitation of this method were 0.04μg / ml and 0.12μg / ml respectively. Butylated sulfonic acid showed a good linear relationship with its peak area in the range of 0.25-8μg / ml, r = 0.999 (n = 7); RSD = 0.31% (n = 6) for the peak area / sample volume with an average recovery of 100.10% and RSD = 1.28% (n = 9). Conclusion The above data prove that the selected column, ion chromatography conditions and determination method for the determination of butane disulfonic acid adenosine methionine succinic acid content of high sensitivity, accurate and reliable, simple and fast.