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通过温室水培试验,研究0.05mmol/LCd2+胁迫及解除Cd2+胁迫对番茄幼苗根系和叶片中抗坏血酸-谷胱甘肽(AsA-GSH)循环代谢的动态影响。结果表明,胁迫条件下,番茄根系和叶片中脂质过氧化产物-丙二醛(MDA)含量增加,细胞膜受损伤;抗坏血酸过氧化酶(APX)活性在胁迫1,3d上升,随后下降,而谷胱甘肽还原酶(GR)活性在胁迫1d时下降,随后上升;抗坏血酸(AsA)和谷胱甘肽(GSH)含量上升,且根系上升幅度大于叶片;解除胁迫后,叶片中MDA含量随时间延长逐渐减少,接近对照,而根系中MDA含量持续上升。APX、GR活性和AsA、GSH含量都能够渐渐恢复,但仍高于对照,根系中GSH含量持续维持较高水平,叶片和根系各参数的恢复程度有差异。
The effects of 0.05mmol / L Cd2 + stress and Cd2 + stress release on the metabolism of AsA-GSH in the roots and leaves of tomato seedlings were studied through hydroponic experiments in greenhouse. The results showed that under stress, the content of lipid peroxidation product - malondialdehyde (MDA) in the roots and leaves of tomato increased and the cell membrane was damaged. The activity of ascorbate peroxidase (APX) increased at 1 and 3 d of stress and then decreased The activity of glutathione reductase (GR) decreased at the 1st day of stress, and then increased. The content of AsA and GSH increased, and the root increased more than the leaves. After the stress was relieved, the content of MDA The time prolonged gradually reduced, close to the control, while the root MDA content continued to rise. APX, GR activities and AsA, GSH contents gradually recovered, but still higher than the control. The content of GSH in roots maintained at a high level, and the recovery of the parameters of leaves and roots was different.