论文部分内容阅读
研究了以黄嘌呤氧化酶-辣根过氧化物酶-苯酚-4-氨基安替比林反应为显色体系测定不同样液中黄嘌呤浓度的新方法。确定该测定方法的最佳反应条件为:黄嘌呤氧化酶(XO)0·32U/mL,辣根过氧化物酶(HRP)7·0U/mL,4-氨基安替比林(AAP)1mmol/L,苯酚(PA)6mmol/L溶于100mmol/LTris-HCl缓冲液(pH8·4);反应温度为37℃,保温时间为20min;检测波长为508nm。本方法测定黄嘌呤浓度的线性范围为0·2～10·0mmol/L,线性关系良好(R=0·9978),检测限为0·05mmol/L。方法操作简单易行,测定结果准确可靠,可有效应用于普通实验室和常规临床血液生化检测。
A new method was developed for the determination of xanthine in different samples with xanthine oxidase - horseradish peroxidase - phenol - 4 - aminoantipyrine as chromogenic reagent. The optimal reaction conditions for the determination of this method were as follows: xanthine oxidase (XO) 0.32 U / mL, horseradish peroxidase (HRP) 7.0 U / mL, and 4 mmol aminoantipyrine (AAP) / L, phenol (PA) 6mmol / L was dissolved in 100mmol / L Tris-HCl buffer solution (pH8.4); reaction temperature was 37 ℃, incubation time was 20min; detection wavelength was 508nm. The linear range of this method for the determination of xanthine was 0.2-10.0 mmol / L, with good linearity (R = 0.9978) and a limit of detection of 0.05 mmol / L. The method is simple and easy to operate, the determination results are accurate and reliable, which can be effectively applied to routine laboratory and routine clinical blood biochemical detection.