丙型肝炎病毒(HCV)感染具有明显的慢性化倾向。与肝细胞癌(HCC)发生关系密切,是肝癌的病因之一.近年来HCV 核心蛋白的作用受到重视,被认为具有基因调节功能.我们采用基因重组技术构建 HCV 核心基因 cDNA真核表达质粒,导入 HepG2细胞株,以期获得永久性表达 HCV核心蛋白的细胞,为进一步探讨 HCV 核心蛋白的致癌机制提 Hepatitis C virus (HCV) infection has a clear tendency toward chronicity. Is closely associated with hepatocellular carcinoma (HCC) and is one of the causes of liver cancer.In recent years, the role of HCV core protein has been taken seriously, and it is considered as a gene regulatory function.We constructed the eukaryotic expression plasmid of HCV core gene by gene recombination technology, Into HepG2 cell line in order to obtain permanent expression of HCV core protein cells, in order to further explore the HCV core protein carcinogenic mechanism