目的 :研究输精管结扎后 3个月及同条件下假手术对照组大鼠小脑和阴茎组织一氧化氮合酶 (NOS) m RNA的表达。方法 :应用 RT- PCR法测定。结果 :1小脑 NOSm RNA表达 :结扎后 3个月组 (VG3)与假手术 3个月组 (SOG3)相比 ,P<0 .0 0 1 ,二者有显著性差异 ,SOG3高于 VG3。 2阴茎 NOS m RNA表达 :VG3与 SOG3相比 ,P<0 .0 5,二者有显著性差异 ,SOG3高于 VG3。结论 :输精管结扎在此时期可影响小脑及阴茎组织中 NOS m RNA表达 ,因而可能造成小脑及阴茎组织一氧化氮 (NO)合成降低 AIM: To investigate the expression of nitric oxide synthase (NOS) mRNA in the cerebellum and penile tissue of rats in sham operation control group 3 months after vasectomy. Methods: The RT-PCR method was used. Results: 1 The expression of NOSmRNA in cerebellum: Compared with SOG3 group, the level of NOSmRNA in VG3 group was significantly lower than that in VG3 group (P <0.01). 2 Penis NOS m RNA expression: VG3 compared with SOG3, P <0. 05, the two were significantly different, SOG3 higher than VG3. Conclusion: Vasectomy can affect the expression of NOS m RNA in cerebellum and penile tissue during this period, which may result in the decrease of nitric oxide (NO) synthesis in cerebellum and penile tissue