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目的:探讨纤溶酶原激活物抑制物-1(PAI-1)基因启动子区4G/5G插入和缺失多态性与反复自然流产的相关性。方法:应用聚合酶链反应(PCR)技术检测57例反复自然流产患者和153例对照组PAI-1基因启动子区4G/5G多态性,并进行比较。结果:反复自然流产组PAI-1基因型频率和等位基因频率分布:4G/4G为26.3%,4G/5G为54.4%,5G/5G为19.3%,4G等位基因频率为0.535,5G等位基因频率为0.465;对照组PAI-1基因型频率和等位基因频率分布:4G/4G为21.6%,4G/5G为56.2%,5G/5G为22.2%,4G等位基因频率为0.497,5G等位基因频率为0.503,经统计学处理,两组各基因型和等位基因频率均无显著性差异(P>0.05)。结论:PAI-1基因启动子区4G/5G多态性与反复自然流产发病无明显的相关性。
Objective: To investigate the relationship between 4G / 5G insertion and deletion polymorphisms of plasminogen activator inhibitor-1 (PAI-1) gene promoter region and recurrent spontaneous abortion. Methods: Polymerase chain reaction (PCR) was used to detect 4G / 5G polymorphism of PAI-1 gene promoter in 57 patients with recurrent spontaneous abortion and 153 controls. Results: The frequencies of genotypes and alleles of PAI-1 in repeated spontaneous abortion group were 26.3% for 4G / 4G, 54.4% for 4G / 5G, 19.3% for 5G / 5G and 0.535, 5G for 5G / 5G The allele frequencies of PAI-1 genotype in control group were 21.6% for 4G / 4G, 56.2% for 4G / 5G, 22.2% for 5G / 5G and 0.497 for 4G allele, The frequency of 5G allele was 0.503. There was no significant difference in genotype and allele frequencies between the two groups (P> 0.05). Conclusion: The 4G / 5G polymorphism of PAI-1 gene promoter region has no obvious correlation with the incidence of recurrent spontaneous abortion.