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含钙培养液(对照)和仅用IAA处理的原生质体的体积和~(45)Ca~(2+)放射性强度均无变化。IAA处理含钙培养液中的原生质体,5min后~(45)Ca~(2+)积累明显增多,体积开始膨大。处理30min时~(45)Ca~(2+)积累最多,此时原生质体的膨大效应最好;随后~(45)Ca~(2+)积累和膨大效应逐渐下降。K~+、Zn~(2+)、Ba~(2+)、Mg~(2+)等也可在一定程度上代替Ca~(2+)使原生质体体积膨大。原生质体的吸水在膨大中起着一定作用。EGTA、LaCl_3和verapamil均抑制IAA诱导的原生质体~(45)Ca~(2+)积累和体积膨大。说明Ca~(2+)可能在6-BA诱导原生质体膨大的过程中起着重要作用。
There was no change in both the volume of Ca2 + -containing (control) and the protoplasts only treated with IAA and the radioactivity of ~ (45) Ca2 +. IAA treatment of protoplasts in calcium-containing culture medium, ~ (45) Ca ~ (2+) accumulation increased significantly after 5min, the volume began to expand. After 30 min, Ca (45) Ca 2+ accumulated most, and the protoplast expansion was the best. At the same time, the accumulation and expansion of ~ (45) Ca 2+ gradually decreased. K ~ +, Zn ~ (2 +), Ba ~ (2 +), Mg ~ (2+) and so on can also replace the Ca ~ (2 +) to a certain extent to protoplast volume expansion. Protoplast water absorption plays a role in the enlargement. Both EGTA, LaCl_3 and verapamil inhibited IAA-induced protoplast ~ (45) Ca ~ (2+) accumulation and volumetric expansion. Ca 2+ could play an important role in the process of protoplast expansion induced by 6-BA.