本文研究了杜氏利什曼原虫前鞭毛体的感染抑制骨髓衍生的巨噬细胞(BMM)发生编程性死亡(Apoptosis)的机制。 从6—8周龄雌性BALB/c小鼠股骨中提取骨髓细胞,细胞在含10％L929细胞条件培养基(LCM)中培养18h,使细胞分化并粘附于聚苯乙烯培养皿上。未粘附的成熟细胞继续培养5天使细胞分化。当细胞处于静止期后18h,用不含Ca~(2+)和Mg~(2+)的冷HBSS In this paper, we investigated the mechanism of the infection of Leishmania donovani promastigotes inhibiting the apoptosis of bone marrow-derived macrophages (BMMs). Bone marrow cells were extracted from the femurs of 6-8 week old female BALB / c mice and cultured in 10% L929 cell conditioned media (LCM) for 18 h, allowing the cells to differentiate and adhere to polystyrene dishes. Non-adherent mature cells were cultured for a further 5 days to differentiate cells. When cells were at rest 18 h, cells were treated with cold HBSS without Ca 2+ and Mg 2+