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将从国外引进的pCD-SRa-huIL-4质粒,在本研究室扩增,纯化,酶切图谱鉴定正确后,用以转染在本室维持增殖的COS 7细胞(经DEAE-Dextran处理),获细胞培养上清液(COS 7 Sup)。按IL-4有支持活化的B细胞及T细胞增殖的作用,检测COS 7Sup中的IL-4活性、经二次SAC-活化人B细胞试
The pCD-SRa-huIL-4 plasmid introduced from abroad was amplified, purified and identified by restriction enzyme digestion. The plasmid pCD-SRa-huIL-4 was transfected into COS 7 cells (DEAE-Dextran treatment) , And cell culture supernatant (COS 7 Sup) was obtained. The activity of IL-4 in COS 7Sup was tested by the effect of IL-4 in supporting the proliferation of activated B cells and T cells. After secondary SAC-activated human B cell test