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目的:建立HPLC法同时测定升麻葛根汤中4种有效成分的含量。方法:采用Diamonsil C_(18)色谱柱(250 mm×4.6mm,5μm),流动相:甲醇-乙腈-[1.6%冰醋酸的0.1 mol·L~(-1)醋酸铵溶液](12:7:81),流速:1.0 ml·min~(-1),检测波长:320 nm。结果:咖啡酸的线性范围为0.2～4 mg·L~(-1)(r=0.999 6),平均回收率为104.0%,RSD为0.59%;葛根素的线性范围为10～200 mg·L~(-1)(r=0.999 5),平均回收率为99.8%,RSD为0.63%;阿魏酸的线性范围为1～20 mg·L~(-1)(r=0.999 5),平均回收率为102.8%,RSD为0.96%;异阿魏酸的线性范围为2～40 mg·L~(-1)(r=0.999 6),平均回收率为98.0%,RSD为1.37%(n=9)。结论:方法简便、快捷、重复性好,可用于该汤剂的质量控制。
Objective: To establish an HPLC method for the simultaneous determination of four active ingredients in Shengmaigeng Decoction. Methods: Diamonsil C_ (18) column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol - acetonitrile - [0.1 mol·L -1 ammonium acetate solution : 81), flow rate: 1.0 ml · min -1, detection wavelength: 320 nm. Results: The linear range of caffeic acid was 0.2-4 mg · L -1 (r = 0.999 6) with the average recovery of 104.0% and the RSD of 0.59%. The linear range of puerarin was 10-200 mg · L ~ (-1) (r = 0.999 5). The average recovery was 99.8% and the RSD was 0.63%. The linear range of ferulic acid was 1 ~ 20 mg · L -1 (r = 0.999 5) The average recoveries were 98.0% and RSDs were 1.37% (n (subscript n)), the recovery was 102.8% and the RSD was 0.96%. The linear range of isoferulic acid was 2-40 mg · L -1 (r = 0.999 6) = 9). Conclusion: The method is simple, rapid, reproducible and can be used for the quality control of the decoction.