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植物和病毒在基因水平上的互作是植物感病和抗病的分子基础。以模式植物烟草及其3种RNA病毒(马铃薯Y型病毒,Potato virus Y,PVY;烟草花叶病毒,Tobacco mosaic virus,TMV;黄瓜花叶病,Cucumber mosaic virus,CMV)为试材,旨在研究受病毒共同诱导、并可能在植物抗病反应中具有重要功能的转录因子基因。试验首先获得10个受病毒诱导的烟草同源基因。在比较了3种病毒侵染、低温和盐害协迫以及逆境信号物质(茉莉酸甲酯与水杨酸)处理对上述基因表达的影响后发现,3种RNA病毒所诱导的植物基因有所不同。但属于WRKY基因家族的06G基因受3种病毒的共同、高效诱导,其表达水平与叶片发育程度呈负相关。研究结果表明,该基因在植物病毒病发生及植物抗病分子育种中可能有重要意义。
Plant-virus interaction at the genetic level is the molecular basis of plant disease and disease resistance. The model plant tobacco and its three RNA viruses (Potato virus Y, PVY; Tobacco mosaic virus (TMV); cucumber mosaic virus (CMV) To study the transcription factor genes that are co-induced by the virus and may play important roles in the plant disease response. The experiment first obtained ten virus-induced tobacco homologous genes. After comparing the effects of three viral infections, hypothermia and salt stress, and the effects of stress signal substances (methyl jasmonate and salicylic acid) on the above gene expression, it was found that the three plant viruses induced by RNA viruses different. However, the 06G gene belonging to the WRKY gene family was jointly and efficiently induced by three viruses, and its expression level was negatively correlated with the degree of leaf development. The results showed that the gene may play an important role in plant virus disease and plant disease-resistant molecular breeding.