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为了解骨髓增生异常综合征(MDS)患者成骨细胞的生物学特性,并探讨其对体外造血的支持能力,利用骨髓间充质干细胞多向分化潜能将其诱导分化为成骨细胞,在体外和造血细胞构建二维培养体系,研究其体外支持造血祖细胞生存的作用;同时采用RT-PCR的方法在mRNA水平上分析由骨髓间充质干细胞诱导分化的成骨细胞细胞因子的表达并与人成骨细胞系hFOB1.19比较。结果发现,来源于MDS患者的成骨细胞在无外源性细胞因子的条件下能够短期维持GM-CFC造血祖细胞的存活。经过RT-PCR证实,人成骨细胞系hFOB1.19表达SCF、IL-6、SDF-1α、G-CSF、GM-CSF等细胞因子,来源于MDS患者和正常人的成骨细胞也能表达上述细胞因子,但却不表达GM-CSF。结论:与正常人相比,MDS患者源的成骨细胞同样能够维持GM-CFC的存活,这可能和成骨细胞表达多种重要的造血相关的细胞因子有关。
In order to understand the biological characteristics of osteoblasts in patients with myelodysplastic syndrome (MDS) and to explore their ability to support hematopoiesis in vitro, the osteoblasts were induced to differentiate into osteoblasts by using the multidrug differentiation potential of bone marrow mesenchymal stem cells. And hematopoietic cells to construct a two-dimensional culture system to study the in vitro survival of hematopoietic progenitor cells; at the same time, the expression of osteoblasts induced by BMSCs was analyzed by RT-PCR at mRNA level and Human osteoblastic cell line hFOB1.19 compared. The results showed that osteoblasts derived from MDS patients were able to maintain the survival of GM-CFC hematopoietic progenitor cells for a short period of time in the absence of exogenous cytokines. The expression of hFOB1.19 in human osteoblast cell line was confirmed by RT-PCR. SCF, IL-6, SDF-1α, G-CSF and GM-CSF were also expressed in osteoblast cell lines. Osteoblasts derived from MDS patients and normal human The above cytokines, but not GM-CSF. CONCLUSION: Osteoblasts derived from MDS patients can also maintain the survival of GM-CFC compared with normal controls, which may be related to the expression of many important hematopoietic cytokines by osteoblasts.