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目的探讨环氧化酶(COX)-2诱导的前列腺素(PG)-E2对白介素(IL)-1α刺激的人牙周膜细胞(PDL)基质金属蛋白酶(MMP)-3表达的调节作用。方法从牙周组织健康个体获得人PDL细胞,分别在使用和不使用消炎痛(COX抑制剂)、NS-398(选择性COX-2抑制剂)、PGE2的情况下用IL-1α刺激人PDL细胞,通过酶联免疫吸附试验(ELISA)实验分析PGE2水平,通过ELISA和酪蛋白明胶酶谱分析分别评价MMP-3水平和酪蛋白水解活性。结果 IL-1α增加了MMP-3和PGE2的产生。尽管消炎痛和NS-398完全抑制了IL-1α诱导的PGE2的产生,这两种药物却相同程度的加强了IL-1α诱导的人PDL细胞中MMP-3的产生。外源性PGE2以浓度依赖的方式降低了IL-1α诱导的MMP-3的产生。结论COX-2诱导的PGE2抑制人PDL细胞中IL-1α刺激的MMP-3的产生。
Objective To investigate the regulatory effect of cyclooxygenase (COX) -2-induced prostaglandin (PG) -E2 on the expression of matrix metalloproteinase (MMP) -3 in human periodontal ligament cells (PDL) stimulated by interleukin (IL) -1α . Methods Human PDL cells were obtained from healthy individuals of periodontal tissue and human PDL cells were stimulated with IL-1α with and without indomethacin (COX inhibitor), NS-398 (selective COX-2 inhibitor), PGE2 Cells were analyzed for PGE2 levels by enzyme-linked immunosorbent assay (ELISA) and MMP-3 levels and casein proteolytic activity were evaluated by ELISA and casein gelatin zymography, respectively. Results IL-1α increased the production of MMP-3 and PGE2. Although both indomethacin and NS-398 completely inhibited IL-1α-induced PGE2 production, both drugs enhanced IL-1α-induced MMP-3 production in human PDL cells to the same extent. Exogenous PGE2 decreases IL-1α-induced MMP-3 production in a concentration-dependent manner. Conclusions COX-2-induced PGE2 inhibits the production of IL-1α-stimulated MMP-3 in human PDL cells.