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目的研究柔红霉素(DNR)对白血病K562细胞细胞凋亡的影响。方法对数生长期K562细胞随机分A、B两组,A组(对照组):加RPM I-1640培养液;B组(DNR组):分别加DNR0.2、2、20μmol/L分别作用1、2、6、24 h后,用流式细胞检测术检测K562细胞的凋亡率。结果在没有药物的作用下,K562细胞存在自然凋亡。DNR组:0.2μmol/L分别作用1、2、6、24 h,细胞凋亡率与对照组相比差异均无统计学意义(P>0.05);2μmol/L作用于K562细胞1 h,与对照组相比差异无统计学意义(P>0.05);作用2、6、24 h,与对照组相比差异均有统计学意义(P<0.01);20μmol/L作用1、2、6、24 h,与对照组相比差异均有统计学意义(P<0.01)。结论DNR能促进K562细胞凋亡,并存在剂量、时间依赖性。
Objective To study the effect of daunorubicin (DNR) on the apoptosis of leukemia K562 cells. Methods K562 cells were randomly divided into groups A and B during logarithmic growth phase. Groups A and B were supplemented with DNR0.2,2 and 20μmol / L, respectively. After 1, 2, 6 and 24 h, the apoptotic rate of K562 cells was detected by flow cytometry. The results in the absence of drugs, K562 cells there is a natural apoptosis. DNR group: 0.2μmol / L for 1, 2, 6 and 24 hours, respectively, the apoptosis rate was not significantly different from the control group (P> 0.05); K562 cells treated with 2μmol / There was no significant difference between the control group and the control group (P> 0.05). The effect was significant at 2, 6 and 24 h (P <0.01) 24 h, compared with the control group, the difference was statistically significant (P <0.01). Conclusion DNR can promote the apoptosis of K562 cells in a dose-and time-dependent manner.