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[目的]建立应激适应的整体动物模型,为研究应激适应的分子机制奠定基础。[方法]利用持续束缚建立应激适应模型,高效液相检测血浆儿茶酚胺;放射免疫法检测血浆皮质醇换算成皮质酮含量,Nagarolsen染色法观察心肌病理变化。[结果]适应组大鼠经过应激后,血浆糖皮质激素、儿茶酚胺的分泌水平,血浆LDH、a-HBDH、CK-MB活性显著低于应激组;Nagarolsen染色和电镜结果表明:适应大鼠应激后,心肌损伤明显弱于应激组。[结论]建立了适应动物模型,适应能够降低应激所造成的心肌损伤。
[Objective] To establish the whole animal model of stress adaptation and lay the foundation for studying the molecular mechanism of stress adaptation. [Method] The model of stress adaptation was built by using persistent strangulation. The plasma catecholamine was detected by HPLC. The plasma Cortisol was measured by radioimmunoassay and corticosterone content was measured. The pathological changes of myocardium were observed by Nagarolsen staining. [Results] The levels of plasma glucocorticoid and catecholamine, plasma LDH, a-HBDH and CK-MB in stress rats were significantly lower than those in stress group after being stressed. Nagarolsen staining and electron microscopy showed that the rats in adaptation group After stress, myocardial injury was significantly weaker than the stress group. [Conclusion] The animal model was established to adapt to reduce the myocardial damage caused by stress.