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目的:对一起食物中毒分离的肠炎沙门菌进行生物学特性和分子分型研究。方法:菌株分离、生化鉴定和血清型确定参照GB/T4789.4-2008方法进行,用VITEK-32全自动微生物鉴定系统检测菌株药物敏感性,用实时荧光PCR法检测沙门菌invA基因,用PFGE方法进行分子分型。结果:17份样品检出10株肠炎沙门菌;经耐药性分析,菌株对氨苄西林、氨苄西林/舒巴坦、头孢唑啉、头孢他啶等15种抗生素均为100%敏感,对呋喃妥因70%耐药;invA基因阳性率为100%;经PFGE分型,10株源于食物中毒患者和涂抹物样品的肠炎沙门菌带型一致,为相同菌株。结论:invA基因可作为沙门菌病快速诊断基因;PFGE是一种非常实用的分子分型方法,能用于沙门菌食物中毒细菌同源性的分析。
Objective: To study the biological characteristics and molecular typing of Salmonella enteritidis isolated from food poisoning. Methods: Strain isolation, biochemical identification and serotype determination were performed according to the method of GB / T4789.4-2008. The drug susceptibility was tested by VITEK-32 automatic microbial identification system. The invA gene of Salmonella was detected by real-time PCR and PFGE Methods Molecular typing. Results: Ten strains of Salmonella enteritidis were detected in 17 samples. According to drug resistance analysis, the antibacterials were 100% sensitive to 15 antibiotics including ampicillin, ampicillin / sulbactam, cefazolin and ceftazidime, Resistant. The positive rate of invA gene was 100%. According to the PFGE typing, the ten strains of S. Enteritidis originated from food-poisoning patients and smear samples were consistent and belonged to the same strain. Conclusion: The invA gene can be used as a rapid diagnostic gene for Salmonella. PFGE is a very useful molecular typing method, which can be used to analyze the homology of Salmonella food poisoning bacteria.