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目的:应用组织芯片技术检测磷酸酶及张力蛋白同源基因(PTEN)、P53及人端粒酶反转录酶(hTERT)在Ⅰ型子宫内膜癌中的表达,为Ⅰ型子宫内膜癌的早期诊断和治疗提供更可靠的理论依据。方法:应用组织芯片技术和免疫组化技术检测Ⅰ型子宫内膜癌和正常子宫内膜组织中P53、PTEN及hTERT的表达,并与传统免疫组化研究结果进行对比分析。结果:组织芯片技术的研究结果与传统免疫组化结果具有较好的一致性(Kappa值>0.40,P<0.05),研究结果可靠性较强。PTEN在Ⅰ型子宫内膜癌中的阳性表达率明显低于正常子宫内膜组织;hTERT和P53在Ⅰ型子宫内膜癌中的阳性表达均高于正常子宫内膜组织。结论:组织芯片技术可作为一种有效的方法应用于Ⅰ型子宫内膜癌研究,PTEN的表达缺失、P53及hTERT的阳性表达与Ⅰ型子宫内膜癌的形成和发展有关,三者均可作为Ⅰ型子宫内膜癌诊断及判定预后的标记物。
Objective: To detect the expression of PTEN, P53 and hTERT in type Ⅰ endometrial carcinoma by tissue microarray technique, which is type Ⅰ endometrial carcinoma The early diagnosis and treatment provide a more reliable theoretical basis. Methods: The expressions of P53, PTEN and hTERT in type Ⅰ endometrial carcinoma and normal endometrium were detected by tissue microarray and immunohistochemistry, and compared with the results of traditional immunohistochemistry. Results: The results of tissue microarray technique were in good agreement with the results of traditional immunohistochemistry (Kappa value> 0.40, P <0.05), and the results were highly reliable. The positive expression rate of PTEN in type Ⅰ endometrial carcinoma was significantly lower than that in normal endometrial tissue. The positive expression of hTERT and P53 in type Ⅰ endometrial carcinoma were higher than that in normal endometrial tissue. Conclusion: Tissue microarray technique can be used as an effective method in the study of type Ⅰ endometrial carcinoma. The expression of PTEN is absent. The positive expression of P53 and hTERT is related to the formation and development of type Ⅰ endometrial carcinoma. As a type I endometrial cancer diagnosis and prognosis of markers.