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[目的]探索一种适合荞麦的简单易行的染色体原位PCR技术。[方法]采用16S套式引物、4.5S套式引物与psbA引物,以栽培甜荞为材料,进行了染色体原位PCR、原位套式PCR与多次原位PCR试验。[结果]高温干燥可以起到与包埋类似的作用;染色体的原位套式PCR效果比原位PCR明显;多次原位PCR次数为5～6效果较佳。16S引物和4.5S引物均显示了4对信号,但位置不同;而psbA引物是单拷贝的,仅显示出1对信号。根据这些信号得位置差异可以区分普通荞麦的5对染色体。[结论]所使用的荞麦染色体原位PCR技术简单易行。
[Objective] The research aimed to explore a simple and convenient chromosome in situ PCR technique suitable for buckwheat. [Method] Chromosome in situ PCR, in situ nested PCR and multiple in situ PCR were carried out using 16S nested primers, 4.5S nested primers and psbA primers, and cultivated sweet buckwheat as material. [Result] The high temperature drying could play a similar role as the embedding. The in situ nested PCR of chromosomes was more effective than in situ PCR. The number of times of in situ PCR was 5 ~ 6 was better. Both the 16S primer and the 4.5S primer showed 4 pairs of signals but at different positions, while the psbA primer was a single copy showing only 1 pair of signals. According to the differences of these signals, we can distinguish 5 pairs of chromosomes of common buckwheat. [Conclusion] The in situ PCR technique of buckwheat chromosome used was simple and easy.