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研究干酪乳杆菌、植物乳杆菌和双歧杆菌在体外降胆固醇能力及三种益生菌混合发酵豆乳对高脂饮食小鼠血脂及肝损伤的影响。采用邻苯二甲醛法评价益生菌体外降胆固醇能力。采用C57BL/6N小鼠,并将其随机分为7组,即对照组;高脂组;干酪乳杆菌发酵豆乳干预组;植物乳杆菌发酵豆乳干预组;双歧杆菌发酵豆乳干预组;三种益生菌混合发酵豆乳干预组;未发酵豆乳干预组。连续喂养7周后,测定血清和肝脏中相关血脂指标,并观察肝脏组织病理学变化。结果表明:干酪乳杆菌、植物乳杆菌和双歧杆菌在体外降胆固醇能力分别为43.14%、46.27%和40.53%。益生菌混合发酵豆乳显著降低小鼠腹部和肾周脂肪指数,血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL),升高高密度脂蛋白(HDL)水平,降低肝脏TC、TG、丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平,并明显改善肝脏脂肪堆积。
To study the ability of Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium to reduce cholesterol in vitro and the effect of three kinds of probiotic mixed fermented soybean milk on the blood lipid and liver injury in high-fat diet mice. The method of o-phthalaldehyde was used to evaluate the ability of probiotics to reduce cholesterol in vitro. C57BL / 6N mice were randomly divided into 7 groups: control group, high fat diet group, lactobacillus fermentum soybean milk intervention group, Lactobacillus plantarum fermented soybean milk intervention group, Bifidobacterium fermented soybean milk intervention group, Probiotic mixed fermented soybean milk intervention group; unfermented soybean milk intervention group. Seven weeks after continuous feeding, serum and liver related serum lipids were measured, and liver histopathological changes were observed. The results showed that the cholesterol lowering ability of Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium in vitro were 43.14%, 46.27% and 40.53% respectively. Probiotics mixed fermented soymilk significantly reduced the abdomen and perirenal fat index, serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) Liver TC, TG, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and significantly improve liver fat accumulation.