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目的探讨PgP和p16蛋白在直肠癌组织中的表达情况及临床意义。方法应用MaxVisionTM免疫组化的方法对71例直肠癌及癌旁组织进行PgP和p16蛋白检测。结果 PgP的总阳性率为84.51%(60/71)。PgP的阳性表达与直肠癌的淋巴结有无转移有关,与癌组织的分化程度、浸润深度与伴不伴有神经浸润无关。其中低分化腺癌(93.33%,28/30)高于高分化腺癌(82.61%,19/23)、中分化腺癌(72.22%,13/18,P>0.05)。淋巴结无转移组93.48%(43/46)明显高于淋巴结转移组68%(17/25,P<0.05)。伴有神经浸润组85.71%(24/28)与不伴有神经浸润组(83.72%,36/43,P>0.05)两者之间差异无统计学意义。癌组织浸润到浆膜外脂肪组织(87.5%,21/24)高于浸润到肠壁全层(84.21%,32/38),高于浸润到肠壁肌层(77.78%,7/9,P>0.05)。p16蛋白的总阳性率为(64.79%,46/71)。p16蛋白的阳性表达与淋巴结有无转移及组织的分化程度无关,与伴不伴有神经浸润及癌组织的浸润深度有关。其中其低分化腺癌(73.33%,22/30)高于高分化腺癌(60.87%,14/23)和中分化腺癌(55.56%,10/18,P>0.05)。淋巴结转移组(17/25,68%)与淋巴结无转移组(29/46,63.04%)两者之间差异无统计学意义(P>0.05)。不伴有神经浸润组(79.07%,34/43)明显高于伴有神经浸润组(42.86%,12/28,P<0.05)。癌组织浸润到肌层(88.89%8/9)高于浸润到全层(71.05%,27/38),明显高于癌组织浸润到浆膜外脂肪组织(45.83%,11/24,P<0.05)。结论 PgP和p16蛋白的阳性表达与直肠癌的某些临床病理指标相关,可作为对判断直肠癌的恶性程度,预测肿瘤侵袭转移,评估预后的良好指标。
Objective To investigate the expression of Pgp and p16 protein in rectal cancer and its clinical significance. Methods The MaxVisionTM immunohistochemical method was used to detect PgP and p16 in 71 cases of rectal cancer and adjacent tissues. Results The total positive rate of PgP was 84.51% (60/71). The positive expression of PgP was related to the metastasis of lymph nodes in rectal cancer, which was not associated with the degree of differentiation, depth of invasion and noninvasive nerve invasion. The differentiated adenocarcinoma (93.33%, 28/30) was higher than that in well-differentiated adenocarcinoma (82.61%, 19/23) and moderately differentiated adenocarcinoma (72.22%, 13/18, P> 0.05). 93.48% (43/46) in lymph node-free metastasis group was significantly higher than 68% (17/25, P <0.05) in lymph node metastasis group. There was no significant difference between the two groups (85.71% (24/28) and without neural invasion group (83.72%, 36/43, P> 0.05). The infiltration into the extra-serous adipose tissue (87.5%, 21/24) was higher than the infiltration to the full thickness of the intestinal wall (84.21%, 32/38) and higher than the infiltration into the intestinal wall muscle (77.78%, 7/9, P> 0.05). The total positive rate of p16 protein was (64.79%, 46/71). The positive expression of p16 protein has nothing to do with the metastasis of lymph nodes and the degree of differentiation of the tissue, but also with the depth of infiltration which is not associated with nerve infiltration and cancer tissue. The poorly differentiated adenocarcinoma (73.33%, 22/30) was higher than that of well-differentiated adenocarcinoma (60.87%, 14/23) and moderately differentiated adenocarcinoma (55.56%, 10/18, P> 0.05). No significant difference was found between lymph node metastasis group (17 / 25,68%) and lymph node metastasis group (29/46, 63.04%) (P> 0.05). The percentage of noninvasive neuronal infiltration group (79.07%, 34/43) was significantly higher than that of the nerve infiltration group (42.86%, 12/28, P <0.05). The infiltration of cancerous tissue into the muscular layer (88.89%, 8/9) was higher than that of infiltrating into the full layer (71.05%, 27/38), significantly higher than that of cancerous tissue (45.83%, 11/24, P < 0.05). Conclusion The positive expression of PgP and p16 protein is correlated with some clinicopathological parameters of rectal cancer, which can be used as a good indicator to judge the malignant degree of rectal cancer and predict the invasion and metastasis of tumor and evaluate the prognosis.