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研究荷瘤小鼠肿瘤组织中髓系抑制性细胞(myeloid-derived suppressor cell,MDSC)的比例变化及分布特点,探讨其在机体抗肿瘤免疫中发挥的作用。采用肝癌细胞原位移植法建立小鼠原位肝癌模型,流式细胞仪检测荷瘤小鼠不同时间肿瘤组织中MDSC的比例;免疫组织化学法检测MDSC在肿瘤组织中的分布情况,以正常小鼠作对照。MDSC的比例在荷瘤小鼠肿瘤组织中比正常小鼠肝组织中明显升高,差别具有统计学意义(P<0.05),且随着荷瘤时间的延长,MDSC的比例逐渐增加。正常小鼠肝组织中仅有极少量MDSC,而荷瘤后大量增加的MDSC主要分布于肿瘤组织与正常组织交界处。可见荷瘤小鼠肿瘤组织中MDSC的比例与肿瘤进展密切相关,提示荷瘤小鼠体内MDSC增多可能参与肿瘤的免疫逃逸,促进肿瘤细胞的生长。
To investigate the proportion and distribution of myeloid-derived suppressor cells (MDSCs) in tumor-bearing mice and to explore their roles in anti-tumor immunity. The in situ hepatocellular carcinoma model was established by orthotopic transplantation of hepatocellular carcinoma cells. The proportion of MDSC in tumor tissues was detected by flow cytometry. The distribution of MDSC in tumor tissues was detected by immunohistochemistry. Mouse as control. The proportion of MDSC in tumor-bearing mice was significantly higher than that in normal mice (P <0.05), and the proportion of MDSC increased with the increase of tumor-bearing time. Normal mouse liver tissue only a very small amount of MDSC, and a significant increase in tumor-bearing MDSC mainly located in the tumor tissue and normal tissue junction. It can be seen that the proportion of MDSC in tumor-bearing mice is closely related to tumor progression, suggesting that increased MDSC in tumor-bearing mice may be involved in the immune escape of tumor and promote the growth of tumor cells.